Table 7.

Average of Ct values and standard deviation of real-time PCR results according to the pretreatment/extraction protocol and to oocyst concentration.

Pretreatment/Extraction Protocol	Average of Ct Values ± Standard Deviation
	100 oocysts/mL	500 oocysts/mL	1000 oocysts/mL
QF	37.60 a ± 1.03	35.99 ± 0.84	35.27 ± 0.84
IN	35.70 b ± 0.94	33.13 ± 0.23	32.66 ± 0.28
MP	34.20 c ± 0.45	33.23 ± 0.44	33.46 ± 0.57
MPG	34.30 ± 0.81	30.86 ± 0.25	31.11 ± 0.48
EM	33.60 ± 0.48	31.53 ± 0.32	30.86 ± 0.31
QP	33.54 ± 0.39	31.48 ± 0.24	29.88 ± 0.20
ZR	31.91 ± 0.18	29.86 ± 0.16	28.89 ± 0.37

^a, average calculated on 9 values of Ct; ^b, average calculated on 16 values of Ct; ^c, average calculated on 15 values of Ct; QF, QIAamp Fast DNA Stool Mini Kit^®, Qiagen; MP, MagnaPure 96^® without grinding, Roche Diagnostics; IN, ELITE InGenius^®, ELITechGroup; MPG, MagnaPure 96^® with grinding, Roche Diagnostics; EM, Nuclisens^® EasyMAG^®, bioMérieux; QP, QIAamp Power Fecal DNA Kit^®, Qiagen; ZR, Quick DNA Fecal/Soil Microbe Miniprep^®, ZymoResearch.