Figure 1.

Scheme of immunization and measure protocol. Subcutaneously immunization of mice was administered four times (days-56, -42, -28 and -14) at a 2-week interval before orally challenged with 300 T. spiralis ML on day 0. At day 5 and 30 after challenge, intestinal adult worm and muscle larval burden (larvae per gram, LPG) were respectively assessed to evaluate the immune protection induced by the rTsT immunization. Histopathological examination of muscle and intestines from infected mice was also performed at day 5 and 30 after larval challenge infection.