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. 2020 Sep 15;9(9):2099. doi: 10.3390/cells9092099

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Flow cytometry data showing the uptake of SiO₂ particles in J774A.1 macrophages. Cells were prestimulated with 1-µg/mL LPS for 24 h and then exposed to a single particle type or a combination of 59-nm SiO₂-BDP FL and 920-nm SiO₂-Cy5 particles. For comparison, we used unstimulated cells exposed only to SiO₂ particles in the same manner. The particles were administered sequentially or simultaneously for different time points (4 h and 24 h). (a) Median fluorescence intensity (MFI) of the BDP FL signal. (b) Median fluorescence intensity (MFI) of the Cy5 signal. Data show a significant increase (* p < 0.05) in the MFI of the BDP FL and Cy5 signals after LPS prestimulation. No significant difference in the MFI was observed between the single- and co-exposure conditions. Control cells were stained with DAPI and were not exposed to SiO₂ particles.

Flow cytometry data showing the uptake of SiO₂ particles in J774A.1 macrophages. Cells were prestimulated with 1-µg/mL LPS for 24 h and then exposed to a single particle type or a combination of 59-nm SiO₂-BDP FL and 920-nm SiO₂-Cy5 particles. For comparison, we used unstimulated cells exposed only to SiO₂ particles in the same manner. The particles were administered sequentially or simultaneously for different time points (4 h and 24 h). (a) Median fluorescence intensity (MFI) of the BDP FL signal. (b) Median fluorescence intensity (MFI) of the Cy5 signal. Data show a significant increase (* p < 0.05) in the MFI of the BDP FL and Cy5 signals after LPS prestimulation. No significant difference in the MFI was observed between the single- and co-exposure conditions. Control cells were stained with DAPI and were not exposed to SiO₂ particles.