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. 2020 Sep 6;12(9):2533. doi: 10.3390/cancers12092533

Figure 2.

Figure 2

Effect of TGFβ on mRNA levels of THBS4, PDGFRβ, and PDGFRβ ligands. Relative mRNA expression levels as determined with real-time polymerase chain reaction (PCR) for (A) PDGFRβ, THBS4, (B) PDGF-A, PDGF-B, PDGF-C, and PDGF-D of DLD-1 cells cultured in the presence (TGFβ 10mM) or absence (control) of TGFβ for 12 h. (C) Western blot with anti-PDGF-D, p-PDGFRβ, and PDGFRβ antibodies in DLD-1 cells cultured in the presence (TGFβ 10 μM) or absence (control) of TGFβ for 12 h. Three independent experiments were performed in duplicate. * p < 0.05 when compared with the control t-test.