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. 2020 Sep 17;8(9):1427. doi: 10.3390/microorganisms8091427

Figure 4.

Figure 4

Blood levels of key cytokines in vaccinated calf groups. (A) IFN-γ response in calves before vaccination (0, −1 month), post vaccination (1, 2 months) and post challenge (2–12 months). Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and stimulated with Johnin PPD for 72 h. IFN-γ levels in culture supernatants were determined using a Bovigam ELISA kit. Data are expressed as an ELISA index, with error bars representing the standard deviation; * p < 0.05 compared to the PBS group. (B) IL-17 response in calves 2 months post vaccination. PBMCs were isolated from whole blood and stimulated with Johnin PPD for 72 h. IL-17 levels in culture supernatants were determined using an ELISA kit. Data are expressed as mean values with error bars representing the standard deviation; * p< 0.05 compared to the PBS group.