Figure 5.

Cytokines release and exhaustion marker expression of iKP-19-CAR-T cells after coculture with Burkitt’s lymphoma Daudi cells. iKP-19-CAR-T cells or CD19-CAR-T cells and Daudi cells were co-cultured in 96 well plate at a 1:1 ratio for 6 h in RPMI complete media. (A) Cell culture media were collected and IL-6, IFN-γ and TNF-α were determined by flow cytometry using CBA assay kit (n = 4 different donors). (B) CAR-T cells were collected and stained with FITC-anti-human PD-1 antibody and PE/Cy7-anti-human TIM-3 antibody to measure the expression of exhaustion markers PD-1 and TIM-3 (n = 4 different donors). MFI was statistically analyzed from three different experiments of each donor. ** p < 0.01, *** p < 0.001. Error bars represent ± SD.