Table 1.
Experimental design for three-factor five-level central composite design as well as comparison between experimental and predicted response values for the growth of the indigenous isolate Enterococcus casseliflavus (Y, optical density at 630 nm) in MRS broth.
| Run | Factor | Response (Experimental 1/Predicted) | ||
|---|---|---|---|---|
| X 1 | X 2 | X 3 | Y | |
| 1 | 7.8 | 10.0 | 47 | 0.175/0.228 |
| 2 | 10.5 | 5.0 | 40 | 1.582/1.490 |
| 3 | 7.8 | 10.0 | 30 | 0.481/0.514 |
| 4 | 5.0 | 15.0 | 20 | 0.107/0.172 |
| 5 | 10.5 | 5.0 | 20 | 1.306/1.281 |
| 6 | 7.8 | 10.0 | 30 | 0.565/0.514 |
| 7 | 7.8 | 10.0 | 30 | 0.579/0.514 |
| 8 | 7.8 | 1.6 | 30 | 1.370/1.356 |
| 9 | 5.0 | 15.0 | 40 | 0.119/0.116 |
| 10 | 7.8 | 18.4 | 30 | 0.152/0.205 |
| 11 | 3.1 | 10.0 | 30 | 0.105/−0.015 |
| 12 | 7.8 | 10.0 | 13 | 0.113/0.099 |
| 13 | 7.8 | 10.0 | 30 | 0.459/0.514 |
| 14 | 5.0 | 5.0 | 20 | 0.125/0.178 |
| 15 | 5.0 | 5.0 | 40 | 0.206/0.271 |
| 16 | 10.5 | 15.0 | 40 | 0.208/0.128 |
| 17 | 7.8 | 10.0 | 30 | 0.599/0.514 |
| 18 | 10.5 | 15.0 | 20 | 0.161/0.068 |
| 19 | 12.4 | 10.0 | 30 | 0.764/0.923 |
| 20 | 7.8 | 10.0 | 30 | 0.410/0.514 |
1 Mean value of three independent measurements (n = 3).