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. 2020 Jun 29;8(3):346. doi: 10.3390/vaccines8030346

Figure 2.

Figure 2

Analysis of BTV-4 NS1 and NS1-Nt protein expression by indirect immunofluorescence assay. Top Row: Detection of recombinant protein in DF-1 cells infected with (a) MVA-wt (negative control), (b) MVA-NS1 or (c) MVA-NS1-Nt at a MOI of 1. Bottom row: Detection of recombinant protein in HEK-293 cells infected with (d) ChAdOx1-wt (negative control), ChAdOx1-NS1 (e) or (f) ChAdOx1-NS1-Nt at a MOI of 1. Viral protein was detected using a hyperimmune serum against BTV-16; nucleus stained with DAPI; Alexa Fluor 594 goat conjugated anti-mouse IgG (red) was used to detect protein in DF-1 cells; Alexa Fluor 488 goat conjugated anti-mouse IgG (green) was used to detect protein in HEK-293 cells. Magnification 400×.