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. 2020 Jun 29;8(3):346. doi: 10.3390/vaccines8030346

Figure 8.

Figure 8

Analysis of protection elicited by ChAdOx1/MVA-NS1 immunization in sheep after infection with BTV-4M. (a) Viremia analyzed by RT-qPCR. Expression of mRNA of segment 5 (encoding NS1 protein) was quantified at 0, 2, 4, 6, 8 and 11 d.p.i. with BTV-4M. Results are expressed as Ct (left y-axis) and PFU equivalents (right y-axis and dotted horizontal lines). Cut-off Ct ≥ 38 (dotted pink line). Differences between groups were calculated by multiple t test analysis sing the Sidak–Bonferroni method. * p-value < 0.033 (b) Mean rectal temperatures responses in ChAdOx1/MVA-NS1 vaccinated and non-vaccinated sheep from 0 to 10 d.p.i. with BTV-4M. The fever threshold (dotted black line) was set to ≥40.50 °C based on the mean plus three standard deviations of the rectal temperatures recorded in the six sheep for one week before challenge. Dots indicate media of the group. Error bars represent SEM. Differences between groups were calculated by multiple t test analysis using the Sidak–Bonferroni method.