Table 1.
Composition of the different culture media used in the androgenesis protocols of Phase 2. The table collects the information on concentrations of basal salts, carbon source, growth regulators and gelling agent for the preparation of media E0, E6, Cb and R. Preparations C and R are specific formulations previously described [12,36].
| Medium | MS + Vitamins (g/L) | Prepared C (g/L) | Prepared R (g/L) | Sucrose (g/L) | Zeatin Riboside (mg/L) | Kinetin (mg/L) | 2,4-D (mg/L) | Vitamin B12 (mg/L) | Gelrite (g/L) | Bacto-Agar (g/L) |
|---|---|---|---|---|---|---|---|---|---|---|
| E0 | 2.20 | - | - | 15.00 | - | - | - | - | 7.00 | - |
| E6 | 2.20 | - | - | 15.00 | 2.00 | - | - | - | 7.00 | - |
| Cb | - | 4.55 | - | 120.00 | - | 5.00 | 5.00 | 0.20 | - | 8.00 |
| R | - | - | 4.55 | 30.00 | - | 0.10 | - | - | - | 8.00 |