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. 2020 Sep 5;9(9):272. doi: 10.3390/biology9090272

Table 5.

SPET mass genotyping results shown as a percentage of heterozygosity (mean and range) calculated based on the genotyping of 532 single nucleotide polymorphism (SNP) positions in the accessions derived from materials BC3 17-19 and BC3 17-4. Among these materials, we find backcrossings with the cultivated parental MEL3 (BC4) and plants obtained from the application of the E6 (DH) protocol. The number of positions SNPs evaluated and those that were not found in the specific accessions are shown.

Accession/Offspring n Missing SNPs Heterozygosity (%)
ELE BC3 17-19 1 0.00 100.00
BC4 (ELE BC3 17-19 × MEL3) 6 4.00 (0.00–6.00) 55.07 (39.06–65.66)
DH (ELE BC3 17-19 doubled haploids) 10 5.00 (0.00–12.00) 1.59 (0.19–3.95)
ELE BC3 17-4 1 0.00 100.00
BC4 (ELE BC3 17-4 × MEL3) 5 0.40 (0.00–2.00) 53.87 (13.35–92.66)
DH (ELE BC3 17-4 doubled haploids) 7 1.14 (0.00–4.00) 0.67 (0.19–1.70)