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. 2020 Jul 20;8(3):399. doi: 10.3390/vaccines8030399

Figure 2.

Figure 2

Figure 2

Figure 2

Gross lesions, histopathological analysis and weight gains, and tissue weight in the safety test. (A) At day 60 after immunization, the tissues of all chickens were collected to observe their gross lesions. (B) At day 60 after immunization, the samples of tissues were fixed in 4% formalin for 24 h and dehydrated, embedded, sliced, and stained with hematoxylin-eosin (HE) for histopathological analysis. (C) At each time point, all chickens were weighed to analyze their weight gains, and at day 60 the tissues of each group were collected to analyze the organ weights (a: body weight; b: tissue weight; NS: no significant difference).