Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 20;8(3):399. doi: 10.3390/vaccines8030399

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

PCR detection and histopathological analysis in the passage test. (A) DNA was extracted from the blood of the first four generations, and PCR was carried out using primers 1 + 4 to detect rMDV; the target gene was 1730 bp (a: first generation; b: second generation; c: third generation; d: fourth generation; 1, 2, 3, 4, 5, 6: chickens of each generation). (B) DNA was extracted from the feathers of the fifth generation, and PCR was carried out using primers 1 + 2, 1 + 3, 1 + 4 to detect rMDV (a: rMDV detection by primers 1 + 2, and the target gene was 827 bp; b: rMDV detection by primers 1 + 3, and the target gene was 1448 bp; c: rMDV detection by primers 1 + 4, and the target gene was 1730 bp). (C) On the 60th day, visceral tissues of the fifth generation were collected for histological examination (a: heart; b: liver; c: spleen; d: lung; e: kidney; f: bursa).

PCR detection and histopathological analysis in the passage test. (A) DNA was extracted from the blood of the first four generations, and PCR was carried out using primers 1 + 4 to detect rMDV; the target gene was 1730 bp (a: first generation; b: second generation; c: third generation; d: fourth generation; 1, 2, 3, 4, 5, 6: chickens of each generation). (B) DNA was extracted from the feathers of the fifth generation, and PCR was carried out using primers 1 + 2, 1 + 3, 1 + 4 to detect rMDV (a: rMDV detection by primers 1 + 2, and the target gene was 827 bp; b: rMDV detection by primers 1 + 3, and the target gene was 1448 bp; c: rMDV detection by primers 1 + 4, and the target gene was 1730 bp). (C) On the 60th day, visceral tissues of the fifth generation were collected for histological examination (a: heart; b: liver; c: spleen; d: lung; e: kidney; f: bursa).