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. 2020 Sep 1;12(9):2477. doi: 10.3390/cancers12092477

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MAS and FAO are major contributors to ATP production in pancreatic cancer cells. Effect of blocking metabolic pathways were analyzed by relative pool sizes of metabolites using targeted LC-MS/MS after various treatments for 24 h. (A) glucose deprivation medium for blocking glycolysis, (B) 5 mM of fluoroacetate (FA) for blocking TCA cycle, (C) 750 µM of amino-oxy acetate (AOA) for blocking MAS system, and (D) 2.5 mM of trimetazidine treatments for blocking FAO in MIA PaCa-2 cells. Data represent the mean and standard deviation of three independent experiments. n.s. (not significant), * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the vehicle control.

MAS and FAO are major contributors to ATP production in pancreatic cancer cells. Effect of blocking metabolic pathways were analyzed by relative pool sizes of metabolites using targeted LC-MS/MS after various treatments for 24 h. (A) glucose deprivation medium for blocking glycolysis, (B) 5 mM of fluoroacetate (FA) for blocking TCA cycle, (C) 750 µM of amino-oxy acetate (AOA) for blocking MAS system, and (D) 2.5 mM of trimetazidine treatments for blocking FAO in MIA PaCa-2 cells. Data represent the mean and standard deviation of three independent experiments. n.s. (not significant), * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the vehicle control.