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. 2020 Sep 1;12(9):2477. doi: 10.3390/cancers12092477

Figure 3.

Figure 3

The TCA cycle is not involved in ATP production in PDAC cells. (A) OCR was analyzed using the Seahorse XFe96 analyzer. OCR in response to 5 mM of FA in MIA PaCa-2, SNU-324, and (B) normal HPNE cells. (C) The levels of OAA and α-KG were measured after treatment of MIA PaCa-2 cells with 5 mM of FA for 24 h using OAA colorimetric/fluorometric assay kit and α-KG colorimetric/fluorometric assay kit. (D) The levels of OAA and α-KG were measured after treatment of HPNE cells with 5 mM of FA for 24 h. Abbreviations: FA, fluoroacetate. OAA, oxaloacetate. α-KG, α-ketoglutarate. n.s. (not significant), * p < 0.05, ** p < 0.01 compared with the vehicle control.