Figure 6.

A high-fat diet increases tumor growth in a human pancreatic cancer xenograft mouse model. (A) MIA PaCa-2 (1 × 10⁷) cells were injected into 6–8-week-old BALB/c nude mice (n = 6 per group) exposed to a HFD or a CD for 12 weeks. (B) Tumor growth was measured using calipers. Tumor size is 2-fold greater in HFD group than in the CD group at 10 weeks. (C) Final weight of subcutaneous tumors derived from MIA PaCa-2 cells were measured. Tumor weight is 2-fold greater in HFD group than in the CD group at 10 weeks. (D) Effect of HFD on body weight in MIA PaCa-2 xenograft mouse model. (E) The levels of NADH and ATP were measured in CD and HFD groups by LC-MS/MS analysis. (F) Effect of HFD on metabolites in MIA PaCa-2 tumor tissues. Relative pool sizes of metabolites determined by targeted GC-MS. FFA, free-fatty acids; CD, control diet; HFD, high fat diet. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the control diet. n.s., not significant.