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. 2020 Sep 7;12(9):2546. doi: 10.3390/cancers12092546

Figure 5.

Figure 5

Doxycycline dose-dependent induction of C/EBPδ regulates proliferation and tumor sphere formation. (A) Single clones of MIA PaCa-2 cells, transduced with a doxycycline-inducible C/EBPδ over-expression plasmid, show increased C/EBPδ mRNA levels upon induction. (B) C/EBPδ induction in MIA PaCa-2 cells over time. The uncropped blots are provided in Figure S3A (C–F) Doxycycline dose-dependently induces C/EBPδ in both transduced MIA PaCa-2 and PANC-1 cells. The uncropped blots are provided in Figure S3B,C. (G,H) Proliferation is mildly decreased by 100 ng/mL and significantly reduced by 2000 ng/mL doxycycline in PANC-1 (** p = 0.0012) and MIA PaCa-2 (** p = 0.0078) cells. (I,J) Tumor sphere formation in soft agar is significantly reduced by 100 and 2000 ng/mL doxycycline in PANC-2 (*** p = 0.0004). A similar yet not significant trend is observed in MIA PaCa-2 with 2000 ng/mL doxycycline. Dox: Doxycycline. n.s.: not significant, *** p < 0.001.