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. 2020 Sep 1;9(9):2005. doi: 10.3390/cells9092005

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transcription factor expression for C2C12 cells at the proliferative stage. (A) Representative images of myogenic transcription factor expression obtained by high content screening (HCS) (scale bar: 100 μM). (B) The average intensity in the nucleus (yellow) and cytoplasm (blue) of every factor at basal (CTRL) and treated conditions. (C) PCA and PL-SDA score plots show the differences and similarities between treatments IL-1β (green), IL-6 (blue), and TNF-α (light blue) and the control (red). (D) Variable importance in projection (VIP) score indicates that MyoD is the most relevant factor at this stage. Data are shown as mean ± SEM (** p < 0.01 vs. control from two-way ANOVA and Dunnett’s post-test; principal component analysis (PCA) and partial least squares discriminant analysis (PSL-DA)).