Figure 5.

Transcription factor expression for C2C12 cells at the commitment stage. (A) Representative images of myogenic transcription factor expression obtained by HCS (scale bar: 100 μM). (B) The average intensity in the nucleus (yellow) and cytoplasm (blue) of every factor at basal (CTRL) and treated conditions. (C) Principal component analysis (PCA) and partial least squares discriminant analysis (PL-SDA) score plots show the differences and similarities between treatments IL-1β (green), IL-6 (blue), and TNF-α (light blue) and the control (red). (D) VIP score indicates myogenin is the most relevant factor at this stage. Data are shown as mean ± SEM (* p < 0.05, ** p < 0.01, and **** p < 0.0001 vs. control from two-way ANOVA and Dunnett’s post-test; PCA and PSL-DA).