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. 2020 Aug 21;9(9):1940. doi: 10.3390/cells9091940

Figure 5.

Figure 5

Levels of LH secretion and AUC after treatment; VH (5 µL DMSO icv plus 200 µL saline iv), RAPA (50 µg rapamycin icv plus 200 µL saline iv), GnRH (5 µL DMSO icv plus 32.5 nmol/kg iv) or RAPA + GnRH (50 µg rapamycin plus 32.5 nmol/kg iv). Administration of the peptides took place after stabilization of the animals following the third sampling point (indicated as time 0). Values are given as the mean ± SEM. p < 0.05; two-way ANOVA repeated measures followed, in case of hormone profile, by a post hoc Bonferroni’s test (groups with different superscript letters are statistically different).