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Cul3 is highly expressed in HIV-1 target cells and not induced by type 1 IFN. (a) Western blot analysis of Cul3 protein levels in HEK293T, Jurkat, THP-1, primary CD4+ T cells and primary macrophages. GAPDH served as loading control. (b) Western blot analysis of Cul3 protein levels in control and IFN-α-A/D-treated (1000 U/mL) THP-1 cells. ISG56 served as positive control and β-actin served as loading control.
