Figure 3.

Mirror biting and predator avoidance behavior endpoint comparisons between the control, 1 ppm, and 2.5 ppm donepezil-exposed zebrafish groups after 14 days of exposure: (A) mirror biting time percentage and (B) longest duration in the mirror side were analyzed in the mirror biting. The swimming trajectories of (C) control, (D) 1 ppm Donepezil-exposed fish, and (E) 2.5 ppm Donepezil-exposed fish for the mirror biting test were recorded and compared (n = 30 for control and 1 ppm groups, n = 29 for 2.5 ppm group). (F) Approaching predator time and (G) average distance to the separator were measured in the predator avoidance test. The swimming trajectories of (H) control, (I) 1 ppm Donepezil-exposed fish, and (J) 2.5 ppm Donepezil-exposed fish for predator avoidance test were recorded and compared (n = 23 for the control group, n = 26 for 1 ppm group, n = 28 for 2.5 ppm group). The data are expressed as the median with interquartile range and were analyzed using the Kruskal–Wallis test followed by Dunn’s multiple comparisons test (####/**** p < 0.001, ns = not significant).