Figure 4.

Social interaction behavior endpoint comparisons between the control group, 1 ppm, and 2.5 ppm donepezil-exposed zebrafish groups after 14 days of exposure: (A) interaction time percentage; (B) longest duration at separator side; (C) average distance to the separator; and (D) average speed were analyzed. The swimming trajectories of (E) control, (F) 1 ppm Donepezil-exposed fish, and (G) 2.5 ppm Donepezil-exposed fish for social interaction tests were recorded and compared. The data are expressed as the median with interquartile range and were analyzed using the Kruskal–Wallis test followed by Dunn’s multiple comparisons test (n = 29 for control and 2.5 ppm groups, n = 30 for 1 ppm group, * p < 0.05, ##/** p < 0.01, ### p < 0.001, #### p < 0.0001).