Table 1.
Bacterial counts (log10 cfu/mL) in the milk samples obtained either by manual expression or pumping in this study and identification of the diverse types of colonies observed in the different agar media where growth was observed.
| Woman | Sample Collection 1 | Agar Media 2 | Bacterial Counts 3 | Bacterial Species 4 |
|---|---|---|---|---|
| W_1 | Manual | CNA | 3.72 ± 0.13 | S. epidermidis; Corynebacterium spp. |
| MRS-Cys | 3.38 ± 0.06 | S. epidermidis; Lc. lactis | ||
| MCK | 3.30 ± 0.13 | A. ursingii | ||
| Pump | CNA | 3.51 ± 0.04 | S. epidermidis; Corynebacterium spp. | |
| (SUD) | MRS-Cys | 3.34 ± 0.16 | S. epidermidis; Lc. lactis | |
| MCK | 3.86 ± 0.08 | A. johnsonii | ||
| W_2 | Manual | CNA | 3.00 ± 0.04 | S. epidermidis |
| MRS-Cys | 3.47 ± 0.14 | S. epidermidis | ||
| MCK | n.d. 5 | - | ||
| Pump | CNA | 3.08 ± 0.11 | S. epidermidis | |
| (SUD) | MRS-Cys | 2.98 ± 0.11 | S. epidermidis | |
| MCK | n.d. | - | ||
| W_3 | Manual | CNA | n.d. | - |
| MRS-Cys | n.d. | - | ||
| MCK | n.d. | - | ||
| Pump | CNA | n.d. | - | |
| (SUD) | MRS-Cys | n.d. | - | |
| MCK | n.d. | - | ||
| W_4 | Manual | CNA | n.d. | - |
| MRS-Cys | n.d. | - | ||
| MCK | n.d. | - | ||
| Pump | CNA | 2.34 ± 0.10 | S. epidermidis | |
| (SUD) | MRS-Cys | 2.30 ± 0.11 | S. epidermidis | |
| MCK | n.d. | - | ||
| W_5 | Manual | CNA | 2.60 ± 0.16 | S. epidermidis |
| MRS-Cys | 3.00 ± 0.16 | S. epidermidis | ||
| MCK | n.d. | - | ||
| Pump | CNA | 2.78 ± 0.11 | S. epidermidis | |
| (SUD) | MRS-Cys | 2.60 ± 0.10 | S. epidermidis | |
| MCK | n.d. | - | ||
| W_6 | Manual | CNA | n.d. | - |
| MRS-Cys | 2.64 ± 0.20 | S. epidermidis; Str. parasanguinis/lactarius; L. salivarius | ||
| MCK | n.d. | - | ||
| Pump | CNA | 2.86 ± 0.07 | S. epidermidis; Str. parasanguinis/lactarius | |
| (SUD) | MRS-Cys | 2.58 ± 0.08 | S. epidermidis; L. salivarius | |
| MCK | n.d. | - | ||
| W_7 | Manual | CNA | 3.48 ± 0.16 | S. epidermidis; Ko. kristinae |
| MRS-Cys | 3.31 ± 0.13 | S. epidermidis; Li. reuteri | ||
| MCK | n.d. | - | ||
| Pump | CNA | 3.33 ± 0.17 | S. epidermidis; Ko. kristinae | |
| (SUD) | MRS-Cys | 3.16 ± 0.21 | S. epidermidis; Li. reuteri | |
| MCK | n.d. | - | ||
| W_8 | Manual | CNA | 3.12 ± 0.21 | S. epidermidis; Str. salivarius |
| MRS-Cys | 2.30 ± 0.13 | S. epidermidis | ||
| MCK | 2.79 ± 0.05 | A. ursingii | ||
| Pump | CNA | 4.03 ± 0.11 | S. epidermidis; Str. salivarius | |
| (OMP) | MRS-Cys | 3.88 ± 0.12 | S. epidermidis | |
| MCK | 2.26 ± 0.07 | A. ursingii | ||
| W_9 | Manual | CNA | 2.76 ± 0.07 | S. epidermidis; Str. parasanguinis/lactarius; Ko. rhizophila |
| MRS-Cys | 2.60 ± 0.16 | S. epidermidis | ||
| MCK | n.d. | - | ||
| Pump | CNA | 3.26 ± 0.14 | S. epidermidis; Str. parasanguinis/lactarius; Ko. rhizophila | |
| (SUD) | MRS-Cys | 2.30 ± 0.08 | S. epidermidis | |
| MCK | n.d. | - | ||
| W_10 | Manual | CNA | n.d. | - |
| MRS-Cys | n.d. | - | ||
| MCK | n.d. | - | ||
| Pump | CNA | 4.38 ± 0.18 | Stn. maltophilia | |
| (OMP) | MRS-Cys | n.d. | - | |
| MCK | 4.87 ± 0.09 | K. oxytoca; Stn. maltophilia |
1 SUD, Single-use device; OMP, own mother’s pump. 2 CNA, Columbia Nadilixic Acid Agar; MRS-Cys, De Man, Rogosa and Sharpe with L-Cysteine agar; MCK, MacConkey agar. 3 Data are means of three independent analysis ± standard deviations. 4 A., Acinetobacter; K., Klebsiella; Ko., Kocuria; Lc., Lactococcus; L., Ligilactobacillus (previously known as Lactobacillus); Li., Limosilactobacillus (previously known as Lactobacillus) S., Staphylococcus; Stn., Stenotrophomonas; Str., Streptococcus. 5 n.d.—not detected.