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Threshold cycle (TC) changes of BTMs over MqPCR analysis on 14 sheep herds in a longitudinal study (two instances of sampling). At first, 42.86% of BTMs were recognized as positive by qPCR, then changed to negative at the second trial. In total, 28.57% were qPCR-positive in both trials. However, the higher concentrations of DNA (1.05 × 102 ng/μL > C < 5.25 × 10−6 ng/μL) corresponding to lower TC values (15–34 cycles) were visible in the second trial.
