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. 2020 Aug 24;8(9):1287. doi: 10.3390/microorganisms8091287

Figure 3.

Figure 3

Immunization of mice with VLP. CVB3-VLP vaccines induced CVB3-specific serum IgG, IgG1-, IgG2a and neutralizing antibody responses in C57BL/6J-mice. (A) IgG, IgG1- and IgG2a ELISA at day 49 post initial vaccination. Antibody titers below 200 were assigned as negative (indicated by horizontal line); (B) CVB3 neutralizing antibody titers in the sera of mice immunized with CVB3-VLP vaccines in samples taken at day 0, 21, 35, 42 and 49 (n = 5). Mean IgG-, IgG1-, IgG2a- and neutralizing antibody titers are indicated by the line ±SEM. The dotted line represents the positive/negative threshold of neutralizing capacity (dilution 1:16) in the virus neutralization assay. * p < 0.05 and ** p < 0.01 compared to control mice or day 0 as determined by non-parametric Kruskal-Wallis test.