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. 2020 Sep 10;11(3):63. doi: 10.3390/jfb11030063

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Efficiency of siRNA-binding to CA-in-DMEM, CA-in-DMB, and HA. After formulation, particles complexed with siRNA were centrifuged, followed by removal of supernatant. The pellets were (A) directly dissolved in 10-mM EDTA or (B) washed with water by another 15-min centrifugation before dissolving in EDTA. Concentrations of the siRNA bound to the particles were calculated from the formula in Section 2.9. CA-in-DMB and HA showed stable and high binding to siRNA. * indicates significant difference at p < 0.05.