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Stability of HBS stored at different temperatures for different durations. (A–C) Absorbance of HA prepared with HBS that was stored at 4 °C, −20 °C and −80 °C for 1, 7 or 30 days, respectively. Two-sample paired t-test performed for statistical analysis. Compared to freshly prepared HA, significantly more particles were formed with HBS stored at 4 °C for 30 days and at −80 °C for 7 and 30 days.
