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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
In vitro tumor microenvironment workflow allowing analysis of tumor cell growth and migration, immunohistochemical staining, and immunoblotting under varying degrees of co-culture conditions (Schematic created with BioRender.com).