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. 2020 Aug 20;9(9):2695. doi: 10.3390/jcm9092695

Figure 4.

Figure 4

Analysis of telomere length, telomerase activity, and the shelterin complex. (A) Assessment of telomere length by T/S ratio in CD4+ and CD8+ T cells of septic patients (grey bar) and controls (white bar). (B) Telomerase activity determined by telomeric repeat amplification protocol (TRAP) assay in CD4+ and CD8+ T cells of septic patients (grey bar) and controls (white bar). (C) mRNA expression of TRET (telomerase reverse transcriptase) and (D) TERC (telomerase RNA component) in CD4+ and CD8+ T cells of septic patients (grey bar) and controls (white bar). mRNA expression of shelterin complex components TRF1 (Telomeric Repeat Factor 1) (E), TRF2 (F), POT1 (Protection of Telomeres 1) (G), and RAP1 (Repressor/Activator protein 1) (H) in CD4+ and CD8+ T cells of septic patients (grey bar) and controls (white bar). mRNA expression is provided as relative expression compared to the housekeeping gene GAPDH. Data are displayed as a box-and-whisker plot representing the 25th and 75th percentiles (bottom and top of the box), the median (band inside the box), and the minimum and maximum of all data (ends of the whiskers) for (A,B) Data are displayed as mean ± SEM for (C–H). Asterisks (*) denote statistical significance, with p < 0.05.