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. 2020 Aug 20;10(9):1208. doi: 10.3390/biom10091208

Figure 1.

Figure 1

Human chorion membrane decellularization. Representative transversal sections of hematoxylin/eosin (H&E) staining of human chorion membrane (HCM) (A) and decellularized HCM (dHCM) (B). Representative scanning electron microscope (SEM) micrographs of dHCM from the reticular layer side (C) and the trophoblast side (D). Representative transversal sections of DAPI staining from HCM (E) and dHCM (F). Double-stranded DNA (dsDNA) quantification in HCM and dHCM (G). Agarose gel electrophoresis of DNA extracted from HCM and dHCM (H). Thickness of air-dried HCM and dHCM measured with a picometer in at least three different sites (I). Top view of HCM (J) and dHCM (K) cut into pieces of 2 × 2 cm. Swelling behavior of dHCM in culture medium and Dulbecco’s phosphate-buffered saline (D-PBS) (L). ** p ≤ 0.01 and *** p ≤ 0.001.