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. 2020 Aug 20;10(9):1208. doi: 10.3390/biom10091208

Figure 2.

Figure 2

dHCM protein characterization. Representative lanes of two different samples from an SDS-PAGE gel of the digested HCM and dHCM (A). Representative dot blot results for collagen type I, collagen type IV, fibronectin and laminin, in both native and dHCM tissues. Digestion buffer without samples was used as a negative control, while the native protein was used as a positive control (B). Immunolocalization of collagen type I, collagen type IV, fibronectin and laminin in representative transversal sections of HCM and dHCM (C). Quantification of soluble collagen (D), insoluble collagen (E) and sulfated glycosaminoglycans (GAGs) (F) content in HCM, dHCM and the trophoblast layer (TL) in μg/mg of dry tissue. ** p ≤ 0.01; *** p ≤ 0.001 and **** p ≤ 0.0001.