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. 2020 Aug 3;8(3):433. doi: 10.3390/vaccines8030433

Figure 3.

Figure 3

SV-specific antibody responses in vivo after second immunization. Mice were subcutaneously immunized with the SV alone, SV plus LNP, SV plus LNP-CpG, or SV plus alum on days 0 and 21. (a) On day 28, levels of SV-specific total IgG, IgG1, IgG2b, and IgG2c in plasma were evaluated by using ELISA. We used 4000- (●), 20,000- (■), and 100,000- (▲) fold diluted plasma samples. (b) Neutralization titers in plasma samples against Cal7 were evaluated. (a,b) n = 5 per group. Data are means ± SD. (a) Significant differences were only analyzed in the 4000-fold-diluted plasma samples. (a,b) † p < 0.05, †† p < 0.01, ††† p < 0.001, and †††† p < 0.0001 vs. group immunized with the SV alone; * p < 0.05, ** p < 0.01, and **** p < 0.0001, as indicated by Tukey’s test.