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. 2020 Aug 3;8(3):433. doi: 10.3390/vaccines8030433

Figure 6.

Figure 6

Hemagglutinin (HA)-specific antibody responses in vivo. Mice were subcutaneously immunized with recombinant HA (rHA) alone, rHA plus LNP, rHA plus LNP-CpG, or rHA plus alum on days 0 and 21. On day 28, the levels of rHA-specific total IgG, IgG1, IgG2b, and IgG2c in plasma were evaluated by using ELISA. We used 160- (●), 800- (■), and 4000- (▲) fold diluted plasma samples. n = 5. Data are means ± SD. Significant differences were only analyzed in the 160-fold-diluted plasma samples. † p < 0.05 and †††† p < 0.0001 vs. group immunized with rHA alone; ** p < 0.01, *** p < 0.001, and **** p < 0.0001, as indicated by Tukey’s test.