Figure 7.

HA-specific T-cell responses in vivo. Mice were subcutaneously immunized with rHA alone, rHA plus LNP, rHA plus LNP-CpG, or rHA plus alum on days 0 and 21. On day 28, splenocytes were cultured in the presence or absence of rHA in vitro. After 1 (for IL-2) or 5 (for IFN-γ and IL-13) days, the levels of IL-2, IFN-γ, and IL-13 were measured by using ELISA. n = 5 per group. Data are means ± SD. ^† p < 0.05 and ^†††† p < 0.0001 vs. group immunized with rHA alone; * p < 0.05, **** p < 0.0001, as indicated by Tukey’s test.