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. 2020 Sep 10;12(9):2588. doi: 10.3390/cancers12092588

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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VEGF-B levels in relationship with HRAS sublocalization. (A) Lipid accumulation as unveiled by Oil Red staining in sections from tumors generated in chick embryos by the indicated HRAS^V12 site-specific constructs. Scale bar = 100 μm. (B) VEGF-B mRNA and protein levels in the tumors generated by the indicated HRAS^V12 site-specific constructs relative to those in control cells. (C) VEGF-B mRNA and protein levels in tumors generated by the indicated cell lines. Data show mean ± SEM from three independent experiments using 8–10 embryos per case. ** p < 0.01 and *** p < 0.001, **** p < 0.0001 by two-tailed unpaired Student t-test.