Table 1.
List of all ether PE structures that were identified in mouse brain lysosomes. Analysis of ether PE lipids by UPLC-HDMSE as described for purified cortical lysosomes from sham and TBI at 1 h after injury. The “O” and “P” in the lipid ID and lipid structure column refer to alkyl ether PE and vinyl ether PE, respectively. ppm = parts per million, tR = chromatographic retention time in minutes, CCS = collisional cross section (Å2), CN = aliphatic chain significance determined using a student t-test, carbon number, DB = number of double bonds in aliphatic chains, ECN = equivalent carbon number, (p > 0.05). All identified ether PE that reached significance were down- *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001. ns = non-significant regulated in TBI exposed lysosomes.
| Positive ion mode | Negative ion mode | Significance | Fold | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lipid ID | m/z | ppm | tR | CCS | m/z | ppm | tR | CCS | CN | DB | ECN | Lipid structure | Sham/TBI | Change | |
| 1 | LPE(P-16:0) | 438.3003 | 5.5 | 1.7 | 209.2 | 436.2855 | 5 | 1.6 | 203 | 16 | 1 | 14 | LPE(P-16:0) | ns | ns |
| 2 | LPE(P-18:1) | 464.3166 | 6.5 | 1.7 | 213.2 | 462.3021 | 6.7 | 1.6 | 207.7 | 18 | 2 | 14 | LPE(P-18:1) | ns | ns |
| 3 | LPE(P-18:0) | 466.3294 | 0.4 | 2.5 | 215.7 | 464.3169 | 5 | 2.4 | 209.6 | 18 | 1 | 16 | LPE(P-18:0) | ns | ns |
| 4 | PE(P-38:6) | 748.5267 | 1.2 | 9 | 271.9 | 746.5186 | 7.5 | 8.9 | 265.5 | 38 | 7 | 24 | PE(P-16:0/22:6) | * (0.0059) | 1.5 |
| 5 | PE(P-36:4) | 724.5266 | 1.4 | 9.7 | 270.7 | 722.5163 | 4.6 | 9.7 | 261.4 | 36 | 5 | 26 | PE(P-16:0/20:4) | *** (0.0026) | 1.6 |
| 6 | PE(O-38:6) | 750.5418 | 1.9 | 9.4 | 274.5 | 748.53 | 1.9 | 9.4 | 266.7 | 38 | 6 | 26 | PE(O-16:0/22:6) | *** (0.0027) | 1.6 |
| 7 | PE(P-40:6) | 776.5583 | 0.8 | 12 | 279.6 | 774.546 | 2.2 | 11.9 | 271.3 | 40 | 7 | 26 | PE(P-18:0/22:6) | * (0.0106) | 1.1 |
| 8 | PE(P-38:4) | 752.5574 | 2 | 12.9 | 277.1 | 750.5453 | 1.3 | 12.9 | 267.3 | 38 | 5 | 28 | PE(P-18:0/20:4) | *** (0.005) | 1.1 |
| 9 | PE(P-42:6) | 804.5886 | 2 | 14 | 287.1 | 802.5741 | 1.9 | 14 | 276.9 | 42 | 7 | 28 | PE(P-20:0/22:6) | ** (0.0077) | 1.5 |
| 10 | PE(P-32:0) | 676.5275 | 0.1 | 12.4 | 265.8 | 674.5113 | 2.5 | 12.4 | 253.7 | 32 | 1 | 30 | PE(P-16:0/16:0) | ns | ns |
| 11 | PE(P-34:1) | 702.5437 | 0.7 | 12.7 | 269.3 | 700.5291 | 0.7 | 12.7 | 258.8 | 34 | 2 | 30 | PE(P-16:0/18:1)/PE(P-18:1/16:0) | * (0.0408) | 1.2 |
| 12 | PE(P-36:2) | 728.558 | 1.2 | 13 | 273.3 | 726.5468 | 3.4 | 13 | 262 | 36 | 3 | 30 | PE(P-18:1/18:1) | ns | ns |
| 13 | PE(P-38:3) | 754.5733 | 1.6 | 13.8 | 278.4 | 752.5599 | 0.1 | 13.8 | 268.6 | 38 | 4 | 30 | PE(P-18:0/20:3) | * (0.0328) | 1.3 |
| 14 | PE(P-40:4) | 780.5873 | 3.7 | 14 | 282.1 | 778.5802 | 5.9 | 14 | 273.5 | 40 | 5 | 30 | PE(P-18:0/22:4) | * (0.0147) | 1.2 |
| 15 | PE(P-34:0) | 704.5575 | 2 | 14.1 | 276.1 | 702.543 | 1.9 | 14.1 | 260.4 | 34 | 1 | 32 | PE(P-18:0/16:0)/PE(P-16:0/18:0) | ns | ns |
| 16 | PE(P-36:1) | 730.5741 | 0.5 | 14.2 | 278.6 | 728.558 | 2.6 | 14.2 | 264.8 | 36 | 2 | 32 | PE(P-18:0/18:1) | ns | ns |
| 17 | PE(P-38:2) | 756.5886 | 2.1 | 14.2 | 281 | 754.5784 | 3.7 | 14.2 | 270.9 | 38 | 3 | 32 | PE(P-18:1/20:1) | * (0.0337) | 1.3 |
| 18 | PE(O-40:4) | 782.6054 | 0.5 | 14.1 | 284.8 | 780.5909 | 0.4 | 14.1 | 274.1 | 40 | 4 | 32 | PE(O-20:0/20:4) | ns | ns |
| 19 | PE(P-38:1) | 758.6039 | 2.5 | 14.7 | 283.6 | 756.5948 | 4.8 | 14.7 | 271.2 | 38 | 2 | 34 | PE(P-18:0/20:1) | ns | ns |
| 20 | PE(P-40:2) | 784.6219 | 0.5 | 14.8 | 286.6 | 782.6074 | 0.6 | 14.7 | 276.1 | 40 | 3 | 34 | PE(P-18:1/22:1) | ns | ns |
| 21 | PE(P-38:0) | 760.62 | 2 | 15.3 | 285.5 | 758.6055 | 1.8 | 15.3 | 272.7 | 38 | 1 | 36 | PE(P-18:0/20:0)/PE(P-20:0/18:0) | ns | ns |
| 22 | PE(P-40:1) | 786.6386 | 1.9 | 15.2 | 287.2 | 784.6241 | 2 | 15.2 | 277.4 | 40 | 2 | 36 | PE(P-18:0/22:1) | ns | ns |