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. 2020 Sep 22;9(9):2143. doi: 10.3390/cells9092143

Table 3.

Template primers for mutagenesis (mutated sites underlined).

MiR-9 binding site 5′-CCAAAGA-3′
3′UTR EFEMP1 MUT Forward 5′-ATAAAATAGTGCTTTAAGGTAACAATATCGTGTCGCTGACTTAAA TGCCTGTGGTTGACTCT-3′
3′UTR EFEMP1 MUT Reverse 5′-AGAGTCAACCACAGGCATTTAAGTCAGCGACACGATATTGTTAC CTTAAAGCACTATTTTAT-3′