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Minimal residual disease (MRD) detection by flow cytometry. (a) Plasma cell identification using CD38 and CD138 combination; (b) very little amount of abnormal plasma cells expressing CD56 (P5), and large amount of normal plasma cells expressing CD19 (P6) were detected; abnormal plasma cells expressing (c) only k light chain, and (d) CD28 and (e) CD117 antigens; normal plasma cells expressing (f) both k and l light chains, and (g) CD27 and (h) CD81 antigens.
