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. Author manuscript; available in PMC: 2021 Jun 30.
Published in final edited form as: Biochem Soc Trans. 2020 Jun 30;48(3):1089–1099. doi: 10.1042/BST20191084

Figure 3. Cellular MAS-DNP of protein structure.

Figure 3.

(A) Preparation of proteins at endogenous levels for MAS-DNP in biological environments. (B) Chemical structure of the trimodal polarizing agent TotaFAM. (C) A fluorescent image confirms the cellular uptake of TotaFAM. (D) 1D 13C spectra of HEK293F cells at <6 K using different radicals. Figures are adapted from references [13, 14] with copyright permission.