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. 2020 Aug 27;10(9):1246. doi: 10.3390/biom10091246

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of ¹³C chemical shift spectra for a DNP-enhanced sample containing 5.5/2.7/2/1 DPPC-d₆₂/DPPC/POPG/cholesterol and 2 mol% K₄ made with buffer containing 10% DMSO and 10 mM AMUPol (red) vs. a DNP spectrum for a control sample made without peptide (black) and an NMR spectrum for a control sample made without polarizing agent (grey). The DNP spectra are an average of 32 transients collected in ~2 min; the control NMR spectrum is an average of 512 transients collected in ~35 min. Spectra are scaled to the lipid -CH₂ resonance in the lipid only sample and no appreciable differences in resolution were detected between the samples.