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. 2020 Sep 3;10(9):1270. doi: 10.3390/biom10091270

Figure 2.

Figure 2

Characterization and proliferation rate of fallopian tube epithelium cells (FTECs) during expansion. (A) Primary culture of FTECs were stained by immunofluorescence with a marker for actively dividing cells (Ki67, red), a basal cell marker (p73, red), and DAPI (blue) 5 and 10 days after incubation in expansion or basal medium. Scale bars: 50 µm. (B,C) Quantitation of Ki67- and p73-positive cells at 5 days and 10 days of culture (analysis of variance (ANOVA) test, n = 7). (D) Doubling of population of primary cultured FTECs grown in expansion or basal medium over time. ** p < 0.01, and *** p < 0.001.