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. 2020 Sep 3;10(9):1270. doi: 10.3390/biom10091270

Figure 5.

Figure 5

Effects of each factor in basal medium on fallopian tube epithelial cell (FTEC) differentiation and self-renewal. (A) FTECs were cultured in the different medium 2 Mix (B27, GlutaMAX), 3 Mix (B27, GlutaMAX, nicotinamide), 4 Mix (B27, GlutaMAX, nicotinamide, SB431542), and 5 Mix (B27, GlutaMAX, nicotinamide, SB431542, EGF), and exposed to ALI culture conditions. EdU (5 µM) was added to each group 48 h before the fixation on ALI culture days 5, 8, and 13. Fixed cells were stained for with anti-Ac-tubulin antibody (green), DAPI (blue), and processed for EdU staining (red). Scale bars: 50 µm. (B) Analysis of time-dependent EdU-positive cells in each culture medium using ALI culture conditions. (C) The number of EdU-positive cells were calculated in each medium on ALI culture days 5, 8, and 13. * p < 0.05, ** p < 0.01, and *** p < 0.001.