Table 1.

Comparison of different culture systems of FTECs.

Species	Isolation	Culture Method	Key Supplements	Passage	Storage	Ciliogenesis	Reference
Porcine	Collagenase IV, DNAse I	Expansion: 2D co-culture with MEF cells, Differentiation: ALI culture	Basal medium, Expansion medium	Yes, 5 passages	Yes	Yes	This study
Human	Pronase, DNAse	ALI culture	2% USG	No	No	Yes	[20,24]
Human	Collagenase A	Co-culture with feeder cells, ALI culture	SCM-6F8 medium contained R-sponding1, Jagged-1, ROCK inhibitor, SB431542, nicotinamide	No	No	Yes	[21]
Porcine	Collagenase 1A	ALI culture	Conditioned medium	No	Yes	Yes	[22,23]
Human	Tissue fragment	Tissue culture on transwells	BSA, Fetuin B, Insulin, Transferrin, Selenium	No	No	/	[33]
Human	Collagenase I	3D culture	Wnt3A, RSPO1, GlutaMAX, B27, N2, EGF, Nicotinamide, Noggin, FGF, ROCK inhibitor, SB431542	Yes	No	Yes	[26]
Mouse	Collagenase A	3D culture	GlutaMAX, B27, EGF, Noggin, FGF, ROCK inhibitor, SB431542, Wnt3A, RSPO1	No	No	Yes	[25]
Human	Collagenase trypsin	2D culture	10% FBS, Insulin	Yes	No	No	[15]
Human	Brushed by Cytobrush	2D culture, 3D culture	15% FBS, EGF, Hydrocortisone, Insulin, Bovine pituitary extract	Yes	No	No	[17]
Human	Pancreatin, trypsin	2D culture	BSA, 2.5% FBS, 2.5% NuSerum	No	No	Little cilia	[16]