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. 2020 Sep 21;12(9):2709. doi: 10.3390/cancers12092709

Figure 4.

Figure 4

Mitochondrial signature induced by BTZ-loaded MSNs in RPMI-8226 and BJhTERT cell lines compared to the free BTZ. The mitochondrial profile of untreated (Control) cancer and normal cells or treated for 1 h with FOL-MSN-BTZ, MSN-FOL and BTZ (as positive control) was assessed 18 h after MSNs exposure using the Seahorse XF-e96 analyzer. Specific perturbations were serially injected at 30 min (Oligomycin), 50 min (FCCP) and 70 min (mix of rotenone and Antimycin A). A schematic tracing of OCR flux in RPMI-8226 (A) and BJhTERT (C) and graphic representation of the basal respiration, proton-leak, ATP-link respiration, maximal respiration and spare respiratory capacity in RPMI-8226 (B) and BJhTERT (D) are reported. (E) ATP profiling production in cancer and normal cells treated as in (A–D). Data are the mean ± SEM of three independent experiments performed in sextuplicate and normalized by protein content (SRB). (*) p < 0.05; (**) p < 0.01; (***) p < 0.001; (****) p < 0.0001.