Figure 8.

Klotho knockdown causes cleavage defects in zebrafish embryos. Images were captured from live video (Supplemental Movie 5) of wild type zebrafish embryos without any treatment (uninjected, black arrow) or after injection with dye (mock, blue arrow), or dye plus Klotho-morpholino. Most of the Klotho-morpholino-injected embryos displayed defective phenotypes, including no cleavage (yellow arrow), cleavage stopped at two cells (red arrow), and asymmetrical cleavages (stars). Some embryos died (purple arrow), and others developed normally (white arrow). (A): One to two cells stage; (B): four to eight cells stage; (C): 32 to 64 cells stage; and (D): around 128 cells stage.