Figure 4.

Growth kinetics of the rAOaV-1 SARS-CoV-2 vaccine and AOaV-1 wt constructs. (A) Vero cells were infected with an MOI 1.0 of rAOaV-1-SARS-CoV-2 or AOaV-1-wt for 2 h (0 h post infection) and were then replaced with media to be incubated and extraction of RNA at 6, 12, 24 and 48 h post infection. The viral copy numbers were calculated from AOaV-1 standard run in parallel. (B) Vero cells were infected with AOaV-1 and total cell lysate was prepared at indicated time points post-infection. These lysates were run for Western blotting using HN antibodies to demonstrate virus replication and alpha tubulin as loading control. (C) Similar to section B, cells were infected with rAOaV-1-SARS-CoV-2 and the expression for the HN and alpha tubulin was measured at indicated time points.