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. 2020 Aug 24;8(3):472. doi: 10.3390/vaccines8030472

Figure 5.

Figure 5

Stability replication of AOaV-1 SARS-CoV-2 vaccine candidate in embryonated chicken eggs. (A) The rAOaV-1-SARS-CoV-2 as well as rAOaV-1-wt were propagated in eggs and were quantified in Vero cells. The titer quantification, as shown in the bar chart, represents comparable replication. Both rAOaV-1-SARS-CoV-2 and rAOaV-1-wt were consecutively propagated in chicken embryonated eggs for 5 passages and the virus titer was quantified in Vero cells. (B) The first and fifth passages for both rAOaV-1-SARS-CoV-2 and rAOaV-1-wt were used to infect Vero cells for 24 h before cell lysis and expression analysis for the HN structural protein of the rAOaV-1.