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. 2020 Aug 27;8(9):1304. doi: 10.3390/microorganisms8091304

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A phylogenetic tree of some selected keratinase genes of different Bacillus species was constructed in MEGA 7 and the evolutionary history was inferred using the unweighted pair group method with arithmetic mean (UPGMA). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The keratinase genes (ker) considered in this study were from; Bm: Bacillus mojavensis; Bl: Bacillus licheniformis; Bme: Bacillus methylotrophicus; Bs: Bacillus subtilis; Ba: Bacillus amyloliquefaciens; Bp: Bacillus Pumilus; Bc: Bacillus circulans; Bt: Bacillus tequilensis; Bth: Bacillus thuringiensis; Bce: Bacillus cereus. The keratinase gene (kerBNK1) from Bacillus sp. Nnolim-K1 is indicated with a squared green tip. The GenBank accession numbers are shown in parenthesis.