Table 3.
Effect of chemical agents on KerBNK1 stability.
| Chemical Agent | Concentration | Residual Activity (%) |
|---|---|---|
| None | - | 100 ± 3.92 |
| EDTA | 5 mM | 0 ± 0 |
| 1,10-phenanthroline | 5 mM | 0 ± 0 |
| PMSF | 5 mM | 96 ± 2.62 |
| Sodium thioglycolate | 5 mM | 76 ± 0.51 |
| Dithiothreitol | 5 mM | 92 ± 0.29 |
| Sodium sulfite | 5 mM | 87 ± 0.65 |
| 2-Mercaptoethanol | 5 mM | 102 ± 4.79 |
| Hydrogen peroxide | 1% (v/v) | 91 ± 2.69 |
| Acetonitrile | 1% (v/v) | 91 ± 0.29 |
| Propan-2-ol | 1% (v/v) | 75 ±0.95 |
| Sodium dodecyl sulfate | 5 mM | 84 ± 1.53 |
| Triton X-100 | 1% (v/v) | 106 ± 2.54 |
| Tween-80 | 1% (v/v) | 119 ± 5.59 |
The values are presented as the mean and standard deviation of triplicate assays.